when the chromosomes lines up in the middle it is called metaphase. after that the chromosomes divide to form sister chromatids then it moves to the opposite poles.
Separation and movement of sister chromatids to the opposite poles as a result of shortening of the spindle fiber. ;
When chromosomes are lined up in the middle of the cell, it means the cell is in metaphase. The next event is anaphase, during which sister chromatids are pulled apart by spindle fibers to opposite poles of the cell. This process is crucial for accurate chromosome distribution during cell division.
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