18) A restriction fragment is
A) A codon
B) A segment of RNA
C) A gene
D) A segment of DNA
19) Which of the following processes is NOT
involved in the production of cDNA?
A) Transcription
B) Translation
C) Reverse transcription
D) RNA processing to remove introns
20) The use of an antibiotic resistance gene on a
plasmid used in genetic engineering makes
A) Direct selection of transformed cells possible
B) The recombinant cell more pathogenic
C) Replica plating possible
D) The recombinant cell unable to survive
21) Which bacterium has been genetically modified
to produce a human protein, gamma interferon?
A) Bacillus thuringiensis
B) Staphylococcus aureus
C) E. coli
D) Pseudomonas fluorescens
22) Where does the Ti plasmid naturally occur?
A) In Thermus aquaticus
B) In Saccharomyces cerevisiae
C) In Bacillus thuringiensis
D) In Agrobacterium tumefaciens
23) If you have inserted a gene in the Ti plasmid, the
next step in genetic engineering is
A) Inserting the Ti plasmid into Agrobacterium
B) Transformation of an animal cell
C) Transformation of E. coli with Ti
D) Splicing Ti into a plant
Answer (1)
The answers include identification of restriction fragments as DNA segments, the non-involvement of translation in cDNA production, the role of antibiotic resistance genes in selection, E. coli as the producer of gamma interferon, the natural occurrence of the Ti plasmid, and the next step in inserting Ti plasmids. These concepts are fundamental in genetic engineering and biotechnology. ;
