Molecular cloning is the laboratory process used to create recombinant DNA. It involves replication of the DNA within a living cell, while there is an another method is Polymerase Chain Reaction (PCR) which replicates DNA in the test tube, free of living cells.
The sticky ends of fragmented DNA are joined with vector DNA.
The process of making recombinant DNA involves cutting DNA fragments with restriction enzymes, creating sticky ends, combining these with vector DNA, sealing the fragments using DNA ligase, and transforming host cells. This technique is essential for applications like gene cloning and genetic engineering. Overall, recombinant DNA technology significantly advances biotechnology and medicine.
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